Résumés disponibles (92) :

SALEH Yara (EA4483 - Jean-Marc LO-GUIDICE)
Session :

 

@mail :  yara.saleh.1992@gmail.com      tél. :  0320626819


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Titre de la communication :
Effect of ultrafine atmospheric particles on the respiratory system in mice
Auteurs (et leurs adresses) de la communication :
Saleh Y 1, Anthérieu S 1, Dusautoir R 1, Sotty J 1, Alleman L 2, Canivet L 1, Perdrix E 2, Platel A 1, Nesslany F 1, Garçon G 1, Lo-Guidice JM 1 1 Univ. Lille, CHU Lille, Institut Pasteur de Lille, EA4483-IMPECS; 2 IMT Lille Douai, SAGE, Douai
Résumé de la communication :
Numerous epidemiological studies show that particular atmospheric pollution constitutes a significant health risk through induction of cardiopulmonary diseases and lung cancers. Atmospheric particles are composed of coarse, fine and ultrafine particles (PM10, PM2.5, and PM0.1 respectively). Ultrafine particles have potentially greater toxicity as they are more abundant and are characterized by their higher surface area per unit than larger particles and can reach the deepest areas in the respiratory system; however, they remain unregulated. Our project aims to highlight the specific respiratory impact of atmospheric ultrafine particles compared to fine particulate matter in a relevant in vivo model of sub-chronic exposure to these pollutants. Quasi-ultrafine (PM0.18) and fine (PM2.5) particles have been collected in the urban industrial zone of Dunkirk in north France during a 7-month campaign. The physico-chemical study of the collected particles shows that there is no major difference in elemental and surface chemical composition between PM0.18 and PM2.5. BALB/c mice were then exposed intranasally to 10µg of PM0.18 or PM2.5 3 times a week. After 1 or 3-month exposure, broncho alveolar lavages (BAL) were performed and lung tissues were harvested for histological, epigenetic and transcriptomic analyses. Cytological analyses show that both types of particulate fractions can be internalized in lung cells. Cellular analyses of BAL and preliminary transcriptomic data (mRNA and miRNA) suggest that PM0.18 induced a stronger lung inflammation and mRNA and miRNA deregulations than PM2.5. Complementary studies are in progress to confirm these first data and to identify the metabolic pathways more specifically associated with the toxicity of ultrafine particles.

SALEH Yara (EA4483 - Jean-Marc LO-GUIDICE)