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@mail :  alejandra.mogrovejovaldivia@etu.univ-lille2.fr      tél. :  03 20 62 69 75

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Titre de la communication :
Development of an anti-inflammatory and anti-bacterial textile based on a multilayer system to treated infected wounds
Auteurs (et leurs adresses) de la communication :
Alejandra MOGROVEJO VALDIVIA, Nicolas TABARY, Feng CHAI, Mickaël MATON, Christel NEUT, Bernard MARTEL, Nicolas BLANCHEMAIN
Résumé de la communication :
Infection of chronic wounds represents a public health problem. It's associated with inflammation and delay of healing process. Silver, in its ionic form (Ag+), is an antibacterial agent and has been proposed as an additive in wound dressings for treating wound infection. Nevertheless, high-dose diffusion of silver into the wound damages the skin and disturbs healing process due to the cytotoxicity of silver. The aim of this work is to develop an innovative silver wound dressing based on a multilayer system, which has antibacterial/analgesic activity but reduces silver diffusion into the wound.

A polyester textile (PET) was functionalized by crosslinked β-cyclodextrin polymer (CD) to make a negatively charged layer (PET-CD). PET-CD was loaded with silver (PET-CD-Ag) by immersing it in a silver sulfate solution (10 g/L) to obtain an antibacterial bioactive layer. The drug-uptake capacity was determined by atomic spectroscopy. In a next step, the multilayer system was built on the PET-CD-Ag; a layer-by-layer (lbl) coating was applied by alternating chitosan (0.5% w/v) as cationic polyelectrolyte and cyclodextrin polymer (0.3% w/v) as anionic polyelectrolyte. A curing process was further applied to stabilize the multilayer system (PET-CD-Ag-PEM), the swelling ratio of which was determined in a solution containing 2.5 mmol/L CaCl2 and 142 mM NaCl respectively.
The cytocompatibility was evaluated with epithelial cells (ATCC-CCL5, L132) according to ISO 10993-5 standard. Furthermore, the PET-CD-Ag-PEM textiles were impregnated in ibuprofen solution. The release profile of Ibu was determined under dynamic condition with a USP4 apparatus (Sotax®, 37°C,35 mL/min) coupled with a UV/Vis spectrophotometer. The antibacterial activity of the bioactive textile was evaluated on S. aureus and E. coli by kill-time method. An agar diffusion test with the same bacteria was performed to observe the diffusion/ non-diffusion of silver out of the textile.

The lbl construction on PET-CD or PET-CD-Ag was linear with the number of layers deposited. The silver loading amount (72 or 12 mg/g) did not affect the linear build-up. After curing, the swelling ratios increased reach a maximum (up to 50%). A good cytocompatibility (> 80%) on the bioactive textile was also shown.
Kill-time test revealed a 6 log reduction of E. coli and a 5 log reduction of S. aureus after 24 hours on PET-CD-Ag. The textiles coated by multilayer system showed bacterial reduction in both strains (3 log). The agar diffusion test demonstrated that the multilayer system may act as a barrier to prevent the diffusion of silver.
The Ibu uptake of PET-CD-Ag-PEM textile was 0.6 mg/cm², and the drug release was prolonged for up to 4 hours.

In conclusion, the wound dressing based on a multilayer system provides a sustained anti-infective therapy with a barrier to limit the silver diffusion into the wound and allowed an extended Ibuprofen release.