Ecole Doctorale
BIOLOGIE SANTE
de Lille

Faculté de Médecine
Pôle Recherche
1 place de verdun
59045 Lille cedex - France
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Calendrier - Soutenances de thèse

LILLE 2 (MED)
LAMBERT Melanie  envoyer un message
vendredi 23 mars 2018
(14h00) - Fac. de Médecine - Pôle Recherche - Salle du Conseil
Etude de l'inhibition fonctionnelle du facteur de transcription HOXA9 par des ligands de l'ADN.
 
résumé (français)
abstract (english)
Unité de recherche : INSERM U1172 éq. 04 (FACTEURS DE PERSISTANCE DES CELLULES LEUCÉMIQUES)
directeur de thèse : Marie-Helene DAVID-CORDONNIER
 
LILLE 2 (MED)
TIAN Lu  envoyer un message
mercredi 28 mars 2018
(14h00) - l'Amphitêatre de l'Institut de Biologie de Lille
Isolation and characterization of mammary cancer stem cells in a transgenic mouse model

Breast cancer is the most common cancer in women worldwide. The isolation and characterization of breast cancer stem cells (CSC) are crucial for understanding cancer biology and revealing potential therapeutic targets. One of the major issues in the study of CSC is the lack of reliable markers. A transgenic mouse model (Tg 11.5kb–GFP) was generated using the 11.5kb s-SHIP (stem-SH2-containing 5'-Inositol Phosphatase) promoter that specifically expressed enhanced green fluorescent protein (GFP) in embryonic and various tissue stem cells. In the mammary gland, previous experiments showed that GFP labels puberty cap cells and pregnancy basal alveolar bud cells, and it has been demonstrated that these mammary GFP+ cells are activated tissue stem cells. In order to determine if s-SHIP promoter expression could also mark mammary cancer stem cells, we generated a bi-transgenic mouse model by crossing Tg 11.5kb-GFP mice with Tg C3(1)/Tag mice. Tg C3(1)/Tag mice express SV40 T antigen under the regulatory control of the rat prostatic steroid binding protein C3(1) gene. In female mice, the transgene is expressed primarily in the mammary gland. Mice develop mammary hyperplasia by 3 months of age with subsequent development of mammary adenocarcinoma by 6 months of age.
Here we show the presence of a rare population of GFP+ cells, which are also CD24+/CD49f+/CD29+ in mammary tumors of female bi-transgenic mice. As compared to GFP- cells, GFP+ cells exhibit both a higher tumor sphere-forming potential, and a higher tumorigenicity when transplanted into SCID and FVB recipient mice. Moreover, upon subsequent transplantation, the GFP+ cells generated heterogeneous tumors that displayed properties similar to the primary tumor. Transcriptomic analysis of these GFP+ vs GFP- cells revealed several differentially expressed genes including one protein implicated in the Notch pathway. In addition, from the murine mammary tumor, I have derived a cell line containing a s-SHIP/GFP+ subpopulation that shows resistance to chemotherapy and radiation. I have further studied this subpopulation and found that synuclein gamma could confer radiation resistance to breast cancer cells. Altogether, these results demonstrate that s-SHIP promoter expression is a marker of mammary CSC that enables their identification and isolation via a single consistent parameter.

résumé (français)
Unité de recherche : UMR CNRS 8161 éq. 04 (CANCER BIOLOGY AND CHEMISTRY)
directeur de thèse : Roland BOURETTE
 
LILLE 2 (MED)
COISNE Augustin  envoyer un message
jeudi 28 juin 2018
(14h00) - Fac. de Médecine - Pôle Recherche - Salle des thèses
Déterminants, mécanismes et conséquences de la dysfonction et du remodelage ventriculaire après remplacement valvulaire aortique à travers le rôle des phénomènes inflammatoires
 
résumé (français)
abstract (english)
Unité de recherche : INSERM U1011 éq. 01 (RÉCEPTEURS NUCLÉAIRES DANS LE SYNDROME MÉTABOLIQUE)
directeur de thèse : David MONTAIGNE
 
LILLE 2 (PHARMA)
MARCEAU Alice  envoyer un message
mercredi 04 juillet 2018
(14h00) - Fac. de Médecine - Pôle Recherche - Salle des thèses
Profil moléculaire des leucémies aiguës myéloïdes pédiatriques
 
résumé (français)
abstract (english)
Unité de recherche : INSERM U1172 éq. 04 (FACTEURS DE PERSISTANCE DES CELLULES LEUCÉMIQUES)
directeur de thèse : Claude PREUDHOMME